Not known Facts About attune flow cytometer
Not known Facts About attune flow cytometer
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Details-enriched characterization of mobile populations. Morphological data from illustrations or photos can include into the richness of flow cytometry details. Such as, the determine demonstrates an otherwise conventional apoptosis assay making use of Annexin V and PI, including cell imaging to characterize cells in Each individual populace to expose morphologically distinct features. These insights could not are actually received from multiplex staining on your own.
As samples are acquired about the Attune CytPix Flow Cytometer, the significant-speed brightfield digicam captures and outlets photographs of detected gatherings at a charge nearly six,000 pictures per next, dependant upon the flow price and picture measurement. For bigger overall flexibility, Attune Cytometric Software program helps you to adjust the image capture frequency as essential and choose a certain gate for graphic seize, if needed.
Mix acoustic focusing technological innovation with higher-parameter Investigation for flexible spectral unmixing and conventional workflows.
Find out new insights into your experimental facts by visualizing populations utilizing mixed fluorescence and morphology knowledge. Our software program interface wizards permit downsampling, concatenation (merged information) and dimensionality applications that happen to be constructed in to the software for an intuitive, streamlined info analysis encounter.
"An image just isn't really really worth 1000 dots, nonetheless it seriously really helps to see visuals. It is excellent to see visuals that correlate with staining. By way of example, AnnexinV-favourable cells which might be morphologically various than healthy cells, or CD14+ monocytes which have been greater and much more textured than CD3+/CD4+/CD14- lymphocytes.
In this article, a seasoned consumer has gated singlets confidently. Soon after analyzing the manual singlet gate, the CytPix picture-derived parameter ParticleCount reveals this gate incorporates much more than four% aggregates.
Optimizing gating technique. Even strong guide singlet gating is mistake-inclined and stays a subjective final decision place in Practically all flow cytometry assays. Imaging can be used to substantiate and change gates to incorporate only one cells of curiosity.
Our experts will style panels in your case as often times you more info may need—gratis, no product purchase wanted.
Early signs of this contamination experienced initially been dismissed as particles. Backgating to find out new populations. Backgating imaged cells over the Attune CytPix Flow Cytometer also lets you use morphological capabilities to find out appealing subpopulations that would not be apparent from flow cytometry facts by yourself.
Consumers can check out function illustrations or photos in genuine-time to substantiate sample high quality, enhance protocols, and report the morphology of each cell population recognized for analysis. Imaging capture also Gains from acoustic concentrating, which really helps to place the cells to ensure that a pointy, centered image is attained.
AI-pushed automated picture Examination With all the Attune Cytometric Program interprets event features into distinct morphology parameters which can be combined with standard fluorescence and scatter parameters to reveal extremely reproducible populace modifications and exceptional subsets.
Software Observe: Automated picture Investigation reduces person-to-consumer variability in flow cytometry gating tactics
Leverage the strength of advanced technological innovation and innovation to speed up your discoveries. Believe in in the Attune Flow Cytometer household to provide the dependability, pace, and accuracy that established your perform aside.
Flexible payment program from Agilent Financial Options assist you to obtain the critical instrumentation you require for speedy venture, or to develop your lab.
Gating on these extended parameters lets you quickly and properly determine populations of curiosity and ensure gating technique with little or no handbook review. The Attune application offers use of condition-of-the-art machine Discovering Assessment resources within an expertly intended consumer interface, which makes it extremely simple to operate.
The Attune Xenith cytometer can be a significant-parameter spectral analyzer, delivering the two spectral unmixing and traditional flow abilities for comprehensive and flexible cell Assessment.
Agilent Worth Guarantee assures you not less than 10 years of instrument use with the day you buy. Usually, We are going to credit rating you Using the residual price of the process towards an upgraded design.
What gave the impression to be a homogeneous populace is definitely a lot more assorted—an insight that needs to be deemed when interpreting final results.